Journal: Frontiers in Neuroscience

Article Title: Expression of Dopamine Receptors in the Lateral Hypothalamic Nucleus and Their Potential Regulation of Gastric Motility in Rats With Lesions of Bilateral Substantia Nigra

doi: 10.3389/fnins.2019.00195

Figure Lengend Snippet: Antibodies used in the immunofluorescent study.

Article Snippet: D2 , Rabbit polyclonal , 1:100 , Alomone/ADR-002.

Techniques:

Journal: Reproductive biomedicine online

Article Title: Social psychogenic stress promotes the development of endometriosis in mouse.

doi: 10.1016/j.rbmo.2016.11.012

Figure Lengend Snippet: Figure 4 – Immmunoreactivity staining of different markers in ectopic lesions in different groups. (A) Representative immunostaining of ADRB2 in endometrium in CONTROL and SHAM mice and in ectopic endometrium in STRESSED and UNSTRESSED mice. ADRB2 and DRD2 immunoreactivity was both seen primarily in glandular epithelial cells and was localized in the cytoplasm. Scale bar = 125 μm. (B) Representative immunostaining of DRD2, VEGF, CD31, CD41, F4/80, PCNA and α-SMA in the ectopic lesions in UNSTRESSED and STRESSED groups. VEGF immunoreactivity was seen primarily in glandular epithelial cells and was localized in the cytoplasm. CD31 immunostainings were seen mostly in vascular endothelial cells. CD41 shows the extent of platelet aggregation and F4/80 immunoreactivity represents the extent of macrophage infiltration. PCNA immunoreactivity was seen both in glandular epithelial cells and stromal cells were localized in the cell nucleus, but the change of immunoreactivity in glandular epithelial cells was more obvious. α-SMA staining were seen mostly in the stromal component of the ectopic lesions. Scale bar = 125 μm. Mice in stressed (STRS) and unstressed (UNSTRS) groups had undergone endometriosis-inducing surgery, while mice the SHAM group underwent non-endometriosis-inducing surgery. Mice in unstressed (UNSTRS), SHAM and control (CTL) groups were not exposed to stress.

Article Snippet: For negative controls, the immunoglobulin G (IgG) from the rabbit serum (Sigma, Darmstadt, Germany) was used instead of primary antibodies against ADRB2, DRD2, VEGF, CD31, CD41, α-SMA and PCNA except for F4/80, for which the IgG was from the rat serum (Boster, Wuhan, China) was used, all used at the same concentration with its corresponding primary antibody.

Techniques: Staining, Immunostaining, Control

Journal: Frontiers in Neuroscience

Article Title: Ankk1 Loss of Function Disrupts Dopaminergic Pathways in Zebrafish

doi: 10.3389/fnins.2022.794653

Figure Lengend Snippet: Drd2 immunohistochemistry in adult zebrafish brain. On the right, drd2 protein distribution in transverse sections of zebrafish brain, ankk1 +/+ and ankk1 27 ins/ 27 ins . On the left, schematic depiction of zebrafish brain, transversal section [adapted from ]. (a–e) Boxes on the schematic depictions represent the region of the brain showed by the corresponding immunohistochemistry on the right; (a’–b”) forebrain drd2 staining; (c’–e”) midbrain drd2 staining. Scale bars: (c”,d”,e’) , 50 mm; (a’–e’) , 100 mm; (b”) , 200 mm. Arrows indicate anti-drd2 positive cells.

Article Snippet: Slides were subsequently incubated with anti-ankk1 Rabbit pAb (A16178, ABclonal), or anti-drd2 Rabbit pAb (A12930, ABclonal), 1:200 in BS, ON at 4°C.

Techniques: Immunohistochemistry, Staining